glut solution  (JEOL)

Journal: Natural Products and Bioprospecting

Article Title: Interaction mechanism between luteoloside and corn silk glycans and the synergistic role in hypoglycemic activity

doi: 10.1007/s13659-024-00428-0

Figure Lengend Snippet: The expression of GLUT-1 in HCT116 cells after 24 h of treatment with CSGs, LUT and CSGs/LUT complexes. a GLUT-1 expression of CSGs/LUT complex (200 μg/mL) on HCT116 cells. b GLUT-1 expression of CSGs (200 μg/mL) and LUT (1.7 and 2.3 mg/mL) on HCT116 cells. Data represent the means ± standard errors of the means of at least three independent experiments; * p < 0.05, ** p < 0.01, and *** p < 0.001

Article Snippet: The GLUT-1 protein solution was fixed on the surface of the Series S CM5 Sensor chip (GE Healthcare) with binding and deionization times of 120 and 150 s, respectively, and then the CSGs solution (25, 50, 100, 200, 400 µg/mL) passed through the sensor surface at a speed of 30 µL/min.

Techniques: Expressing

Journal: Natural Products and Bioprospecting

Article Title: Interaction mechanism between luteoloside and corn silk glycans and the synergistic role in hypoglycemic activity

doi: 10.1007/s13659-024-00428-0

Figure Lengend Snippet: The affinity between LUT, CSGs/LUT complexes and Glut-1. a The sensorgram of LUT interacts with GLUT-1 protein at different concentrations. b–e The sensorgram of CSGs/LUT complexes interacts with GLUT-1 protein at 25, 50, 100, 200, and 400 µg/mL

Article Snippet: The GLUT-1 protein solution was fixed on the surface of the Series S CM5 Sensor chip (GE Healthcare) with binding and deionization times of 120 and 150 s, respectively, and then the CSGs solution (25, 50, 100, 200, 400 µg/mL) passed through the sensor surface at a speed of 30 µL/min.

Techniques: